Antisense Technology: Applications Part B by Abelson J.N., Simon M.I., Phillips I.M.

Biology

By Abelson J.N., Simon M.I., Phillips I.M.

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7 injected plasmid with interleukin 5 (IL5) cDNA into the soleus muscle of mice, resulting in increased serum levels of IL-5 and an increased percentage of eosinophils in white blood cells, 2 weeks after injection. Aihara and Miyazaki8 increased IL-5 levels by 100fold by electroporation of genes into the DNA injection site. Decrouy et al. 9 injected tibialis anterior muscles from mdx4cv mice (animal model for Duchenne muscular dystrophy) with plasmid DNA encoding either fulllength or minidystrophin.

Pierce, R. D. Alvarez, G. P. Siegal, J. T. Douglas, and D. T. Curiel, Clin. Cancer Res. 4(10), 2455 (1998). 40 T. J. Wickham, P. W. Roelvink, D. E. Brough, and I. Kovesdi, Nature Biotechnol. 14(11), 1570 (1996). 41 M. m. Croyle, E. Walter, S. Janich, B. J. Roessler, and G. L. Amidon, Hum. Gene Ther. 9(4), 561 (1998). 42 M. M. Burcin, G. Schiedner, S. Kochanek, S. Y. Tsai, and B. W. O'Malley, Proc. Natl. Acad. Sci. A. 96(2), 355 (1999). 43 S. Kochanek, P. R. Clemens, K. Mitani, H. H. Chen, S. Chan, and C.

42 M. M. Burcin, G. Schiedner, S. Kochanek, S. Y. Tsai, and B. W. O'Malley, Proc. Natl. Acad. Sci. A. 96(2), 355 (1999). 43 S. Kochanek, P. R. Clemens, K. Mitani, H. H. Chen, S. Chan, and C. T. Caskey, Proc. Natl. Acad. Sci. A. 93(12), 5731 (1996). 44 It has been used successfully for delivering antisense R N A against a-globin 45 and human immunodeficiency virus (HIV) long terminal repeats, 46 and it is our vector of choice for delivering antisense targeted to the AT1 receptor in cell culture and hypertensive rat models.

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