Amino acids analysis Protocols by Catherine Cooper

Molecular Biology

By Catherine Cooper

A set of vintage and state-of-the-art strategies of excessive application in answering particular organic questions about amino acids. universal tools comprise these in line with HPLC or fuel chromatography separation and research after precolumn derivatization. New ideas according to capillary electrophoresis separation, high-performance anion alternate chromatography, and mass spectrometry also are offered. each one strategy is defined in step by step element to make sure profitable experimental effects and emphasizes pattern instruction, rather the gathering and garage of physically fluids. updated and hugely functional, Amino Acid research Protocols deals analytical and medical chemists, in addition to a vast diversity of organic and biomedical investigators, a wealthy compendium of laboratory instruments for the efficient research of either universal and unusual amino acids.

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Mix of quaternary/tertiary ammonium groups. This mix of functionalized groups has a controlled ratio, resulting in excellent selectivity for the separation of amino acids. The small particle size of the anion–exchange layer exhibits adequate mass transfer characteristics enabling high chromatographic peak efficiency. The highly crosslinked core of the AminoPac PA10 packing makes it possible to use organic solvents to facilitate column cleanup. Use chemicals exactly as specified by catalog number and supplier.

Packer, and K. , Totowa, NJ 63 64 Type Amino acid Alditol Monosaccharide Hydroxy-amino acid Peptide Amino acid Ox. Product of Met Amino acid Peptide Monosaccharide Amino-sugar Amino-sugar Monosaccharide Amino acid Amino acid Monosaccharide Amino acid Amino acid Monosaccharide Monosaccharide Monosaccharide Peptide Amino acid Peptide Amino acid Peptide Amino acid Ox. Product of Met Compound 29 Glycine 30 Ribose 31 Valine 32 Hydroxy-Proline 33 Gly-OHPro 34 Serine 35 Sucrose 36 Gly-Ser 37 Proline 38 Isomaltose 39 IsoLeucine 40 Leucine 41 Methionine 42 NorLeucine 43 Taurine 44 Histidine 45 Phospho-Arginine 46 Diaminopimelic 47 Phenylalanine 48 Glutamate 49 Aspartate 50 Cystine 51 Phospho-Serine 52 Phospho-Threonine 53 Tyrosine 54 Phospho-Tyrosine 55 Cysteic Acid 56 Tryptophan Type Amino acid Monosaccharide Amino acid Hydroxy-amino acid Peptide Amino acid Disaccharide Peptide Amino acid Disaccharide Amino acid Amino acid Amino acid Amino acid Amino sulfonic acid Amino acid Phospho-amino acid Amino acid Amino acid Amino acid Amino acid Amino acid Phospho-amino acid Phospho-amino acid Amino acid Phospho-amino acid Ox.

And Harris, R. J. (1998) Hydroxylation of Lys residues reduces their susceptibility to digestion by trypsin and lysyl endopeptidase. Anal. Biochem. 258, 136–137. 23. Harris, R. J. and Spellman, M. W. (1993) O-Linked fucose and other post-translational modifications unique to EGF modules. Glycobiol. 3, 219–224 24. Yan, S. , Chao, Y. , and van Halbeek, H. (1993) Novel Asn-linked oligosaccharides terminating in GalNAcβ(1→4)[Fucα(1→3)]GlcNAcβ(1→•) are present in recombinant human Protein C expressed in human kidney 293 cells.

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