Aging Methods and Protocols (Methods in Molecular Medicine) by Yvonne A. Barnett

Molecular Biology

By Yvonne A. Barnett

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And McCormick, F. (1996) Kinetic and biochemical correlation between sustained p44ERK1 (44 kDa extracellular signal-regulated kinase 1) activation and lysophosphatidic acid-stimulated DNA synthesis in Rat-1 cells. Biochem. J. 320, 237–245. 58. Lavoie, J. , G, L. , and Pouyssegur, J. (1996) Cyclin D1 expression is regulated positively by the p42/p44MAPK and negatively by the p38/HOGMAPK pathway. J. Biol. Chem. 271, 20608–20616. 59. Meloche, S. (1995) Cell cycle reentry of mammalian fibroblasts is accompanied by the sustained activation of p44mapk and p42mapk isoforms in the G1 phase and their inactivation at the G1/S transition.

Trypsinization and seeding of flasks should be performed in a sterile environment (see Note 8). To propagate adherent cells: 1. 25%) and place it on ice; prepare fresh growth medium and warm it to 37°C. 2. Using sterile procedures in a laminar flow hood, remove spent growth medium from the culture vessel. For flasks and bottles, the medium should be removed by aspiration or decanting from the side opposite the cell growth surface. For cell culture plates and dishes, the medium should be removed by aspiration from the edge of the growth surface.

Littlefield, J. , and Soeldner, J. S. (1969) Diabetes mellitus and aging: diminished plating efficiency of cultured human fibroblasts. Proc. Natl. Acad. Sci. USA 64, 155–160. 72. Martin, G. M. (1978) Genetic syndromes in man with potential relevance to pathobiology of aging, in Genetic Effects on Aging (Bergsma, D. and Harrison, D. ), Alan Liss, New York, pp. 5–39. 73. Danes, B. S. (1971) Progeria: a cell culture study on aging. J. Clin. Invest. 50, 2000–2003. 74. Schneider, E. L. and Epstein, C.

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